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DR Zhiguang Xiao

Positions

IT Access, Florey Department of Neuroscience and Mental Health

Bioinorganic chemistry (Metal-containing proteins and enzymes)
Electrochemistry (Protein electrochemistry)
Inorganic chemistry (Coordination chemistry)
Medicinal chemistry (Metallodrugs)
Synthetic chemistry (Probes for bio-metals)

Overview

Has subject area

For 2008 6 Digit Code
Seo 2008 6 Digit Code
- Expanding Knowledge in the Biological Sciences
- Expanding Knowledge in the Chemical Sciences

Affiliation

Member of

Royal Australian Chemical Institute. C Chem MRACI 2003 -

Publications

Selected publications

2019
- Journal Articles
  - Molecular Mechanisms of Glutaredoxin Enzymes: Versatile Hubs for Thiol-Disulfide Exchange between Protein Thiols and Glutathione. Journal of Molecular Biology. 431. 2019
2018
- Journal Articles
  - Evaluation of Cu(I) binding to the E2 domain of the amyloid precursor protein - a lesson in quantification of metal binding to proteins via ligand competition. Metallomics: integrated biometal science. 10. 2018
  - di-Cysteine motifs in the C-terminus of plant HMA4 proteins confer nanomolar affinity for zinc and are essential for HMA4 function in vivo. 2018
- Journal Articles Refereed
  - Glutaredoxins employ parallel monothiol-dithiol mechanisms to catalyze thiol-disulfide exchanges with protein disulfides. Chemical Science. 9. 2018
  - The Human Amyloid Precursor Protein Binds Copper Ions Dominated by a Picomolar-Affinity Site in the Helix-Rich E2 Domain. Biochemistry. 57. 2018
2017
- Journal Articles Refereed
  - Copper ATPase CopA from Escherichia coli: Quantitative Correlation between ATPase Activity and Vectorial Copper Transport. Journal of the American Chemical Society. 139. 2017
  - Copper binding and redox chemistry of the A beta 16 peptide and its variants: insights into determinants of copper-dependent reactivity. Metallomics: integrated biometal science. 9. 2017
  - Molecular Aspects of a Robust Assay for Ferroxidase Function of Ceruloplasmin. Inorganic Chemistry. 56. 2017
  - Reduction potentials of protein disulfides and catalysis of glutathionylation and deglutathionylation by glutaredoxin enzymes. Biochemical Journal. 474. 2017
2016
- Journal Articles Refereed
  - Evaluation of employing poly-lysine tags versus poly-histidine tags for purification and characterization of recombinant copper-binding proteins. Journal of Inorganic Biochemistry. 162. 2016
  - Metal binding to the N-terminal cytoplasmic domain of the P-IB ATPase HMA4 is required for metal transport in Arabidopsis. Plant Molecular Biology. 90. 2016
2015
- Journal Articles
  - CopC Protein from Pseudomonas fluorescens SBW25 Features a Conserved Novel High-Affinity Cu(II) Binding Site. Inorganic Chemistry. 54. 2015
- Journal Articles Refereed
  - A set of robust fluorescent peptide probes for quantification of Cu(II) binding affinities in the micromolar to femtomolar range. Metallomics: integrated biometal science. 7. 2015
  - The functional roles of the three copper sites associated with the methionine-rich insert in the multicopper oxidase CueO from E. coli. Metallomics: integrated biometal science. 7. 2015
2014
- Journal Articles Refereed
  - An integrated study of the affinities of the A-beta16 peptide for Cu(I) and Cu(II): implications for the catalytic production of reactive oxygen species. Metallomics: integrated biometal science. 6. 2014
  - Glutaredoxin1 protects neuronal cells from copper-induced toxicity. Biometals. 27. 2014
  - Quantification of Copper Binding to Amyloid Precursor Protein Domain 2 and its Caenorhabditis elegans Ortholog. Implications for Biological Function. Metallomics: integrated biometal science. 6. 2014
  - Redox Sulfur Chemistry of the Copper Chaperone Atox1 is Regulated by the Enzyme Glutaredoxin 1, the Reduction Potential of the Glutathione Couple GSSG/2GSH and the Availability of Cu(I). Metallomics: integrated biometal science. 6. 2014
- Conference Proceedings
  - New Probes for Weaker Cu(I) Binding Sites Completes a Set of Four That Can Detect Affinities from Nanomolar to Attomolar. Journal of Biological Inorganic Chemistry. 2014
2013
- Journal Articles Refereed
  - Evaluation of quantitative probes for weaker Cu(I) binding sites completes a set of four capable of detecting Cu(I) affinities from nanomolar to attomolar. Metallomics: integrated biometal science. 5. 2013
  - Interaction of cisplatin and analogue Pt(en)Cl2 with the copper metallo-chaperone Atox1. Metallomics: integrated biometal science. 5. 2013
2012
- Journal Articles Refereed
  - Copper complexes of a novel non-innocent quadridentate ligand. Chemical Communications. 48. 2012
  - PcoE - A metal sponge expressed to the periplasm of copper resistance Escherichia coli. Implication of its function role in copper resistance. Journal of Inorganic Biochemistry. 115. 2012
  - The challenges of using a copper fluorescent sensor (CS1) to track intracellular distributions of copper in neuronal and glial cells. Chemical Science. 3. 2012
2011
- Journal Articles Refereed
  - Metallo-Oxidase Enzymes: Design of their Active Sites. Australian Journal of Chemistry. 64. 2011
  - Molecular Basis of the Cooperative Binding of Cu(I) and Cu(II) to the CopK Protein from Cupriavidus metallidurans CH34. Biochemistry. 50. 2011
  - Unification of the Copper(I) Binding Affinities of the Metallo-Chaperones Atx1, Atox1 and Related Proteins: Detection Probes and Affinity Standards. Journal of Biological Chemistry. 286. 2011
  - Water soluble bis(thiosemicarbazonato)copper(II) complexes. Australian Journal of Chemistry. 64. 2011
2010
- Journal Articles Refereed
  - A Water-Soluble Bis(thiosemicarbazone) Ligand. A Sensitive Probe and Metal Buffer for Zinc. Inorganic Chemistry. 49. 2010
  - Challenges of Determining Metal-Protein Affinities. Natural Product Reports . 27. 2010
  - Reaction Mechanisms of the Multicopper Oxidase CueO from Escherichia coli Support Its Functional Role as a Cuprous Oxidase. Journal of the American Chemical Society. 132. 2010
  - Solvation Effects on S K-Edge XAS Spectra of Fe-S Proteins: Normal and Inverse Effects on WT and Mutant Rubredoxin. Journal of the American Chemical Society. 132. 2010
2009
- Journal Articles Refereed
  - Interaction of Cisplatin and Analogues with a Met-Rich Protein Site. Journal of Biological Inorganic Chemistry. 14. 2009
  - Metal binding affinities of Arabidopsis zinc and copper transporters: selectivities match the relative, but not the absolute, affinities of their amino-terminal domains. Biochemistry. 48. 2009
  - Metal specificities of Arabidopsis zinc and copper transport proteins match the relative, but not the absolute, affinities of their N-terminal domains. Chemical Communications. 42. 2009
  - Unprecedented Binding Cooperativity between CuI and CuII in the Copper Resistance Protein CopK from Cupriavidus metallidurans CH34. Implications from Structural Studies by NMR Spectroscopy and X-ray Crystallography. Journal of the American Chemical Society. 131. 2009
2008
- Journal Articles Refereed
  - Copper resistance in E-coli: The multicopper oxidase PcoA catalyzes oxidation of copper(I) in (CuCuII)-Cu-I-PcoC. ChemBioChem. 9. 2008
  - Electron paramagnetic resonance characterization of the copper-resistance protein PcoC from Escherichia coli. Journal of Biological Inorganic Chemistry. 13. 2008
  - Protonless C-13 direct detection NMR: Characterization of the 37 kDa trimeric protein CutA1. Proteins - Structure, Function, and Bioinformatics. 70. 2008
  - Transfer of copper between bis(thiosemicarbazone) ligands and intracellular copper-binding proteins. Insights into mechanisms of copper uptake and hypoxia selectivity. Inorganic Chemistry. 47. 2008
2007
- Journal Articles Refereed
  - Conserved Mechanism of Copper Binding and Transfer. A Comparison of the Copper-Resistance Proteins PcoC from Escherichia coli and CopC from Pseudomonas syringae. Inorganic Chemistry. 46. 2007
  - Copper and Alzheimer's disease. Current Opinion in Chemical Biology. 11. 2007
2006
- Journal Articles Refereed
  - Intermolecular Transfer of Copper Ions from the CopC Protein of Pseudomonas syringae. Crystal Structures of Fully Loaded CuICuII Forms. Journal of the American Chemical Society. 128. 2006
2005
- Journal Articles Refereed
  - CopC Protein from Pseudomonas syringae: Intermolecular Transfer of Copper from Both the Copper(I) and Copper(II) Sites. Inorganic Chemistry. 44. 2005
2004
- Journal Articles Refereed
  - C-Terminal domain of the membrane copper transporter Ctr1 from Saccharomyces cerevisiae binds four Cu(I) ions as a cuprous-thiolate polynuclear cluster: sub-femtomolar Cu(I) affinity of three proteins invlolved in copper trafficking. Journal of the American Chemical Society. 124. 2004
2003
- Journal Articles Refereed
  - Application of NMRD to hydration of rubredoxin and a variant containing a (Cys-S)3FeIII(OH) site. Biophysical Journal. 84. 2003
- Conference Proceedings
  - Metal binding in CopC from P-syringae pathovar tomato. Journal of Inorganic Biochemistry. 2003
  - The C-terminal domain of the membrane copper pump Ctr1 in yeast binds Cu as a cuprous-thiolate polynuclear cluster. Journal of Inorganic Biochemistry. 2003
2002
- Research Book Chapters
  - Determinants of the reduction potential in Rubredoxins, the simplest iron-sulfur electron-transfer proteins. Modern coordination chemistry: The legacy of Joseph Chatt. Royal Society of Chemistry. 2002
- Journal Articles Refereed
  - A C-Terminal Domain of the Membrane Copper Pump Ctr1 Exchanges Copper(I) with the Copper Chaperone Atx1. Chemical Communications. 2002. 2002
  - Removal of a cysteine ligand from rubredoxin: assembly of Fe2S2 and Fe(S-Cys)3(OH) centres. Journal of Biological Inorganic Chemistry. 7. 2002
2001
- Journal Articles
  - Redox thermodynamics of mutant forms of the rubredoxin from Clostridium pasteurianum: identification of a stable Fe-111 (S-Cys)(3)(OH) centre in the C6S mutant (vol 6, pg 638, 2001). Journal of Biological Inorganic Chemistry. 6. 2001
  - Rubredoxin mutant proteins with Fe-III(S-CYS)(3)(OH) centres. Journal of Inorganic Biochemistry. 86. 2001
- Journal Articles Refereed
  - Redox thermodynamics of mutant forms of the rubredoxin from Clostridium pasteurianum: identification of a stable FeIII(S-Cys)3(OH) centre in the C6S mutant. Journal of Biological Inorganic Chemistry. 6. 2001
2000
- Journal Articles
  - Mutation of the surface valine residues 8 and 44 in the rubredoxin from Clostridium pasteurianum: solvent access versus structural changes as determinants of reversible potential. Journal of Biological Inorganic Chemistry. 5. 2000
1999
- Journal Articles
  - Rubredoxin from Clostridium pasteurianum. Structures of G10A, G43A and C10VG43A mutant proteins. Mutation of conserved glycine 10 to valine causes the 9-10 peptide link to invert. Acta Crystallographica Section D - Biological Crystallography. 55. 1999
  - The dependence of reversible potentials on the form of modification of edge plane pyrolytic graphite electrodes in voltammetric studies on rubredoxin and ferredoxin from Clostridium pasteurianum. Electrochemistry Communications. 1. 1999
- Conference Proceedings
  - Molecular engineering at the active site of rubredoxin. Journal of Inorganic Biochemistry. 1999
1998
- Journal Articles
  - The rubredoxin from Clostridium pasteurianum: Mutation of the iron cysteinyl ligands to serine. Crystal and molecular structures of oxidized and dithionite-treated forms of the Cys42Ser mutant. Journal of the American Chemical Society. 120. 1998
1996
- Journal Articles
  - A catalytic cycle related to molybdenum enzymes containing (MoO2)-O-VI(2+) oxidized active sites. Inorganic Chemistry. 35. 1996
  - Complexes containing cis-(MoO2)-O-V(+) and cis-(MoO)-O-V(OH)(2+) centers. Journal of the American Chemical Society. 118. 1996
  - Dioxomolybdenum(VI) complexes of tripodal nitrogen-donor linands: Syntheses and spectroscopic, structural, and electrochemical studies, including the generation of EPR-active molybdenum(V) species in solution. (vol 34, pg 5954, 1995). Inorganic Chemistry. 35. 1996
  - Rubredoxin from Clostridium pasteurianum: Mutation of the Conserved Cysteine and Glycine Residues. ACS Symposium Series. 653. 1996
  - The rubredoxin from Clostridium pasteurianum: Mutation of the conserved glycine residues 10 and 43 to alanine and valine. Inorganic Chemistry. 35. 1996
  - Trimethylsilyloxo complexes of oxomolybdenum(V) . Journal of Biological Inorganic Chemistry. 1. 1996
  - Trimethylsilyloxo complexes of oxomolybdenum(V) coupled electron-electrophile transfer reactions. Journal of Biological Inorganic Chemistry. 1. 1996
- Conference Proceedings
  - Rubredoxin from Clostridium pasteurianum: Mutation of the conserved cysteine and glycine residues. ACS Symposium Series. 1996
1995
- Journal Articles
  - DIOXOMOLYBDENUM(VI) COMPLEXES OF TRIPODAL NITROGEN-DONOR LIGANDS - SYNTHESES AND SPECTROSCOPIC, STRUCTURAL, AND ELECTROCHEMICAL STUDIES, INCLUDING THE GENERATION OF EPR-ACTIVE MOLYBDENUM(V) SPECIES IN SOLUTION. Inorganic Chemistry. 34. 1995
1994
- Journal Articles
  - ISOVALENT AND MIXED-VALENT DINUCLEAR MU-OXO AND MU-OXO MU-DISULFIDO COMPLEXES OF MOLYBDENUM(V) AND MOLYBDENUM(VI). Inorganic Chemistry. 33. 1994
  - THE CIS-DIOXOMOLYBDENUM(V) RADICAL-ANION, (HB(ME(2)C(2)N(3))(3))MOO2(SPH)(-). Journal of the Chemical Society, Chemical Communications. 1994
1992
- Journal Articles
  - A SINGLE MODEL DISPLAYING ALL THE IMPORTANT CENTERS AND PROCESSES INVOLVED IN CATALYSIS BY MOLYBDOENZYMES CONTAINING MOVIO22+ ACTIVE-SITES. Journal of the American Chemical Society. 114. 1992

Research

Investigator on

Grant
- Molecular study of copper-promoted ubiquitination (Discovery Projects) awarded by AUST RESEARCH COUNCIL 2019 - 2021

Awards

Education and training

MSc, Fujian Normal University
PhD, La Trobe University
BSc, Fujian Normal University

Linkages

Geographic focus

Australia

Supervision

Available for supervision

Y

Contact Details
- z.xiao@florey.edu.au
- 9035 6072
- Location
  - Level: 05 Room: 522, Bio21 Incubator Building, Parkville
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