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DR Paul Veith

Positions

  • Polymicrobial biofilms (subgingival plaque)
  • Protein Secretion (porphyromonas gingivalis)
  • Proteomics (mass spectrometry)

Overview

OverviewText1

  • **RESEARCH INTERESTS**
     
    Protein secretion in pathogenic bacteria
    Chronic periodontitis (gum disease) is associated with specific bacterial pathogens such as Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia. P. gingivalis is the most important of these and produces large quantities of proteases (called gingipains) that are essential for the ability of this pathogen to cause disease. Our recent data indicates that these gingipains are located on the outside of the cell within a special surface layer that can be seen by electron microscopy (Chen et al., 2011). We are intensively studying the secretion system responsible for transporting these gingipains across the outer membrane and incorporating them into this surface layer. This secretion system is newly discovered and seems to be conserved in the bacteroidetes phylum and therefore linked to a variety of diseases and environmental issues. We use a range of techniques including mass spectrometry, electron microscopy, cell fractionation, native and 2D electrophoresis, protein expression, mutant generation, bioinformatics, etc in order to identify the components of this novel secretion system and to determine the role of each component in the secretion mechanism. 

    • Proteomics / Mass Spectrometry

    We have our own well-established proteomics laboratory located in the Bio21 Institute that comprises MALDI-TOF/TOF and Ion Trap mass spectrometers, sample preparation robots, 2D electrophoresis equipment, fluorescent scanner, & in-house proteomics servers (Mascot, Proteinscape). Protein identification projects we have conducted include studies of all three periodontopathogens (Veith et al., 2002; Veith et al., 2009a; Veith et al., 2009b). Apart from having a high capability for all our protein identification needs, we have conducted several quantitative analyses in order to de   

Publications

Selected publications

Research

Investigator on

Awards

Education and training

  • PhD, University of Melbourne 2003
  • BSc(Hons), University of Melbourne 1995
  • BSc, University of Melbourne 1994

Linkages

Supervision

Available for supervision

  • Y