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DR MIRELLA DOTTORI



Contact Details

Organization: Medicine, Dentistry and Health Sciences
Position: RESEARCH FELLOW
Email:
Work: +61 3 8344 3988
Room: 707
Level: 07
Building: Medical Centre
Campus: Parkville

Biography

Dr Dottori is a neurobiologist who undertook her PhD studies in Prof Andrew Boyd’s Lab at WEHI. Her studies were on the Eph family of receptor tyrosine kinases (Lackmann et al., 1998, J Biol Chem; 47 Citations)(Dottori et al., 1999 Blood; 17 Citations). A major part of her studies involved generating a strain of mice lacking the EphA4 receptor. These mutant mice have locomotor defects due to a neural deficit in the corticospinal tract, the major motor neuron tract in the mammalian nervous system (Dottori et al., 1998, Proc. Natl. Acad. Sci. USA; 93 Citations). The EphA4 mutant mice are have been since extensively used by several groups around the world to study neural circuitry, axon guidance, and Eph receptor signaling.
Dr Dottori pursued her interests to study the developing nervous system and undertook her postdoctoral training in Prof Martyn Goulding’s lab at the Salk Institute (La Jolla, CA, USA). Her postdoctoral studies were on early patterning within the neural tube, in particular identifying genes involved in specifying neural crest progenitors (Dottori et al., 2001, Development; 68 Citations) (Gross et al., 2002, Neuron; 71 Citations).
Upon returning to Australia in 2003, Dr Dottori joined Prof Martin Pera’s lab at Monash University, which one of the world leading laboratories of human embryonic stem cell (hESC) research. At this time, many of the techniques were not yet established for hESC, including their genetic modification. Thus, much of Dr Dottori’s initial research was to develop such techniques (Costa and Dottori et al., 2007, Nature Protocols). This work included the generation of a hESC cell line, ‘ENVY” that constitutively expresses green fluorescent protein (Costa and Dottori et al., 2006, Nature Methods). The ENVY hESC cell line is well characterized at genomic level and is a very useful research tool particularly for transplantation studies.
Dr Dottori is currently pursuing her research on neural differentiation of hESC. Her pursuits in this area led to understanding the early differentiation and neural patterning that occurs in neurospheres derived from hESC (Davidson et al., submitted). Her discoveries encouraged her to extend her research more towards understanding the signals involved in neural differentiation from hESC, as this research is very important if hESC derivatives are to be therapeutically used for treatment of disease, such as in neurodegenerative disorders. Dr Dottori’s expertise in stem cell biology has been highlighted with her recent co-authorships (together with Prof Martin Pera) on two book chapters.
Dr Dottori will continue her research, at the University of Melbourne, where she has established her own Stem Cell Lab within the Neural Regeneration group, headed by Dr Turnley. Dr Dottori will bring her expertise in hESC biology to the Centre and her studies in neural stem cell biology will be complimented by working in a very strong neuroscience research environment.

Research Expertise and International Linkages

Research Expertise

Research Interest Country of Expertise
Developmental Neurobiology Australia
Stem Cell Biology Australia

Qualifications, Honours, Fellowships and Other Awards

Qualifications

Title Institution Date Awarded Abbreviation
DOCTORATE IN MEDICAL BIOLOGY University of Melbourne 31-Dec-1998
BACHELOR OF SCIENCE (Hons) University of Melbourne 31-Dec-1992

Government Research Classifications

Research Fields, Courses and Discipline Classifications

Socio-Economic Objective Classifications

Grants and Contracts

Research Grants, Contracts and Consultancies awarded to the University of Melbourne as the administering institution (since 2003) as recorded in Themis Agreements.

Grants

Title Role Funding Source Scheme Award Date
Effect of lysophosphatidic acid and sphingosine-1-phosphate in neuronal stem cells. Chief Investigator NHMRC Project Grants 01/01/2007
Derivation of peripheral sensory neurons from human embryonic stem cells Chief Investigator FRIEDREICH ATAXIA RESEARCH ASSOC (AUSTRALASIA) Research Projects 01/07/2007
GENERATION OF INDUCED PLURIPOTENT STEM CELLS FROM FRIEDREICH ATAXIA PATIENTS Chief Investigator FRIEDREICHS ATAXIA RESEARCH ALLIANCE General Research Grant 01/01/2009
Genetic selection of human dopaminergic neural progenitor cells to optimize transplantation therapies for Parkinson's disease Principal Supervisor RAMACIOTTI FOUNDATION Establishment Grants 01/01/2009
GENERATION OF INDUCED PLURIPOTENT STEM CELLS FROM FRIEDREICH ATAXIA PATIENTS Chief Investigator FRIEDREICH ATAXIA RESEARCH ASSOC (AUSTRALASIA) Research Projects 01/01/2009
Stem cell therapy towards the treatment of genetic and acquired diseases Chief Investigator REBECCA L COOPER MEDICAL RESEARCH FOUNDATION LTD Research Grants

Contracts

Title Role Funding Source Award Date
HUMAN NEURAL PROGENITOR CELL TRANSPLANTATION THERAPY AND HUNTINGTON'S DISEASE. Chief Investigator UNIVERSITY OF AUCKLAND 01/06/2006
DISCOVERY ENGINE FOR EMBRYONIC STEM CELL Chief Investigator UNIVERSITY OF MELBOURNE,
AUST STEM CELL CENTRE LTD (ASCC)
01/01/2007
CONFIDENTIALITY AGREEMENT WITH AUSTRALIAN STEM CELL CENTRE Chief Investigator AUST STEM CELL CENTRE LTD (ASCC)

Additional Grant and Contract Information

Awards:
1992 HECS Exemption Scholarship for Honours, University of Melbourne
1993 - 1996 Australian Postgraduate Research Scholarship
1999 - 2001 Human Frontiers Science Program Organization (International)
Long-Term Postdoctoral Fellowship
2003 - 2005 NHMRC Howard Florey Centenary Fellowship

Publications

Publications produced at the University of Melbourne and reported in the Annual Publications Collection and 'Research Report' since 2001. The Themis Publications module, released in November 2006, allows additional publications from previous institutions and publications from past years to be entered.

Publications in 2009

Book Chapters

Publications in 2008

Book Chapters

Journal Articles

  • Lysophosphatidic acid inhibits neuronal differentiation of neural stem/progenitor cells derived from human embryonic stem cells
    Year: 2008
    Journal: Stem Cells
    Volume: 26
    Page numbers: 1146-1154
    Author(s):
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Publications in 2007

Book Chapters

  • Neural differentiation of human embryonic stem cells
    Year: 2007
    Book: Methods in Molecular Biology: Neural Stem Cells
    Publisher: Humana Press
    Authors(s):

Journal Articles

  • A Method for genetic modification of human embryonic stem cells using electroporation.
    Year: 2007
    Journal: Nature Protocols
    Volume: 2
    Issue: 4
    Page numbers: 792-796
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  • Wnt3a regulates survival, expansion, and maintenance of neural progenitors derived from human embryonic stem cells.
    Year: 2007
    Journal: Molecular and Cellular Neurosciences
    Volume: 36
    Issue: 3
    Page numbers: 408-15
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Publications in 2006

Journal Articles

  • Gap junctions modulate apoptosis and colony growth of human embryonic stem cells maintained in a serum-free system
    Year: 2006
    Journal: Biochemical and Biophysical Research Communications
    Volume: 344
    Page numbers: 181-188
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Publications in 2005

Book Chapters

  • Stem cells and their development potential
    Year: 2005
    Book: Stem Cells From Bench to Bedside
    Publisher: World Scientific Publishing Co
    Authors(s):
    Editors(s):

Journal Articles

  • Neural stem cells express non-neural markers during embryoid body co-culture.
    Year: 2005
    Journal: Stem Cells
    Volume: 24
    Issue: 4
    Page numbers: 918-927
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  • The hESC line Envy expresses high levels of GFP in all differentiated progeny.
    Year: 2005
    Journal: Nature Methods
    Volume: 2
    Issue: 4
    Page numbers: 259-260
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Publications in 2002

Journal Articles

  • EphA4 is not required for Purkinje cell compartmentation
    Year: 2002
    Journal: Developmental Brain Research
    Volume: 135
    Issue: 1-2
    Page numbers: 29-38
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  • Lbx1 specifies somatosensory association interneurons in the dorsal spinal cord.
    Year: 2002
    Journal: Neuron
    Volume: 34
    Page numbers: 535-549
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Publications in 2001

Journal Articles

  • Kinase-dependent and kinase-independent functions of EphA4 receptors in major axon tract formation in vivo.
    Year: 2001
    Journal: Neuron
    Volume: 29
    Page numbers: 73-84
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  • The winged-helix transcription factor Foxd3 suppresses interneuron differentiation and promotes neural crest cell fate.
    Year: 2001
    Journal: Development
    Volume: 128
    Page numbers: 4127-4138
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